Microbiology: Viable Counts

Introduction Total Viable itemize is a numeric idea about the presence of microorganisms such as bacterium, yeast and mold in a sample. It counts the quash of colonies produced by a in truth dilute suspension of bacteria on an agar collection main office and to observe the differential scrap behaviour of the living bacteria. This involves counting the colonies produced by possible cells at a lower place favourable growth conditions. approximately techniques needed before the viable count, like pour plate regularity, spread plate method and most probable identification sum up method. The viable count is very specidic, as it represents the number of dependence forming units (/g) or (/ml) of the sample. B) VIABLE COUNTS Two methods of acquire viable counts are available i. Spread plate method. ii. Most probable number (MPN) method. (i) Spread Plate order (Lawn Culture) Materials: Dilution series prepared in (A) Sterile 1ml pipette 6 intellectual nourishment agar (NA) plates 1 crackpot field hockey stick 1 beaker of alcohol Procedure All move should be through using aseptic technique The culture tagged 10-8 was mildly mixed. 0.1ml of this dilution was aseptically transferred onto the center of a NA plate.

Bend end of the crackpot spreader was dipped in alcohol, then it was sterilized by flaming and allowed to unruffled down. The glass hockey stick was utilise to spread the sample over the aerofoil of the plate. It was an even distribution. This offset printing was repeated using a second NA plate. These p lates were labeled as 10-8. So, duplicated p! late was counted. Steps 1,2 and 3 was repeated for dilution 10-7 and 10-6 in order. 6 plates was prepared and 2 at each of the dilution 10-8, 10-7 and 10-6. The plates were incubated at 32oC for about 2 days. The number of colonies on... If you want to get a wide of the mark essay, order it on our website: OrderCustomPaper.com

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